ABSTRACT
Objective To observe the effect of salted processing on the contents of total alismol,24-acetylalisol A and 23-acetylalisol B in A lisma Rhizoma.Methods UV was used for the determination of total alismol′s content in crude A lisma Rhizoma and salted A lisma Rhizoma with Meta Nitrobenzene and Potassium Hydroxide as the developers.The detection wave-length was 545 nm.HPLC was used for the determination of 24-acetylalisol A′s and 23-acetylalisol B′s contents in crude A lis-ma Rhizoma and salted A lisma Rhizoma with Elite C18(4.6 mm×250 mm,5 μm)as the chromatographic column.The mobile phase was gradient elution with acetonitrile and water.The detection wavelength was 208 nm.The column temperature was set at 25℃.The flow rate was 1 ml/min.Results The linear relationships of total alismol,24-acetylalisol A and 23-acetylalisol B were fine(r>0.999 0)in the linear ranges by UV and HPLC.The average recoveries of them in crude A lisma Rhizoma and salted A lisma Rhizoma ranged from 97.81%-100.05%,with the RSD less than 3%.The average contents of total alismol,24-acetylalisol A and 23-acetylalisol B in ten batches of crude A lisma Rhizoma were 4.41%,0.208%,0.065% respectively.The average contents of them in ten batches of salted A lisma Rhizoma were 9.62%,0.232%,0.017% respectively.Conclusion The contents of total alismol,24-acetylalisol A and 23-acetylalisol B in A lisma Rhizoma were influenced considerably by salted processing.The content of total alismol increased obviously.The content of 24-acetylalisol A increased slightly.The content of 23-acetylalisol B decreased obviously.